403 Forbidden: Access is Denied - Hosting MVC 3 Project - asp.net-mvc-3

I am trying to host MVC Project and this Error Show :
403 - Forbidden Access Is Denied
You do not have permission to view this directory or page using the credentials that you supplied
I
1-Added Virtual application
2-Changed the application pool to ASP.NET v4.0
3-Added Permissions ( IIS Users ) gave it full controll
4-Changed the Identity to LocalSystem
5-Tested my ConnectionString and its SQL Authentication atm with SuperAdmin
That worked on my laptop as local but when i tried to do that on my Server it gives the previous Error

If you're using Windows/Forms Authentication in your application, make sure that you enable the correct option under the Authentication section (IIS7)

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https://www.visualstudio.com/en-us/docs/integrate/api/wit/wiql
this is i am putting in my Postman
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Postman image
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Make sure you have enable Basic Auth for your TFS:

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