Temp file not being deleted - bioinformatics

Question

I'm trying to create a temporary file in my pipeline, then use that file in another rule.
For example, I have two rules in a .smk file:
#Unzip adapter trimmed fastq file
rule unzip_fastq:
input:
'{sample}.adapterTrim.round2.fastq.gz',
output:
temp('{sample}.adapterTrim.round2.fastq')
conda:
'../envs/rep_element.yaml'
shell:
'gunzip -c {input[0]} > {output[0]}'
#Run bowtie2 to align to rep elements and parse output
rule parse_bowtie2_output_realtime:
input:
'{sample}.adapterTrim.round2.fastq'
output:
'rep_element_pipeline/{sample}.fastq.gz.mapped_vs_' + config["ref"]["bt2_index"] + '.sam'
params:
bt2=config["ref"]["bt2_index_path"], eid=config["ref"]["enst2id"]
conda:
'../envs/rep_element.yaml'
shell:
'perl ../scripts/parse_bowtie2_output_realtime_includemultifamily.pl '
'{input[0]} {params.bt2} {output[0]} {params.eid}'
{sample}.adapterTrim.round2.fastq is used once and should ultimately be deleted upon completion. However, I'm finding that this file is uploaded to Amazon S3, even with the addition of temp(). I'm also finding that this file is removed locally, but still persists on S3.
Am I doing this correctly? '{sample}.adapterTrim.round2.fastq' is not currently written in the rule-all of the Snakefile.
We ultimately need to prevent this file from being uploaded to S3, so if there is a way to specify not to upload this file in the rule, that would be useful.

Answer 1

It seems that the snippet in the question is not consistent with actual use, since for S3 files one would need to wrap file names in remote.
However, as a general solution, documentation contains the following:
The remote() wrapper is mutually-exclusive with the temp() and protected() wrappers.
Hence, if you intend to use a temp file, make sure it's not wrapped in remote, or explicitly wrap the file in local.